By Malcolm T Sanford
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Extra resources for 4H - Beekeeping Basic Manual
Nucleic Acid and Peptide Aptamers: Methods and Protocols, vol. com 33 34 Mosing and Bowser Fig. 1. Schematic of the CE–SELEX process. A random sequence DNA library is incubated with the target. Sequences bound to the target are separated using capillary electrophoresis, PCR amplified and made single stranded, generating a new pool suitable for further rounds of enrichment. decreases the time requirement of the process compared to preexisting methods. The CE–SELEX process is illustrated in Fig.
9. Load the gel with each 15 ml of the samples. 10. 5 h. 11. Take the gel out of the gel-running unit and remove one of the glass plates. Cover the gel with plastic foil. 12. Put the gel on a paper towel and put it in an X-ray film cassette. 13. Apply a phosphor-screen and close the cassette and expose the screen for 10 min. g. Fuji FLA 3,000). 9. Filter-Retention Analysis 1. Dilute the radioactive labelled DNA 1:10 with water. 2. The diluted DNA is mixed 1:20 with binding-assay mix. 3. 625 mM by mixing each one part protein solution with one part 1x PBS.
3. Solution of streptavidin-binding buffer containing 10 mM Tris–HCl, 50 mM NaCl, 1 mM ethylenediamine tetraacetic acid (EDTA). Store at room temperature. 4. 15 M NaOH. Store at 2–8°C. 5. 15 M acetic acid. Store at 2–8°C. 5. Ethanol Precipitation 1. Solution of 100% ice cold ethanol. Store at –20°C. 2. Solution of ice cold 70:30 ethanol/water. Store at –20°C. 3. Solution of 3 M sodium acetate. Store at room temperature. 3. Methods The methods outlined in this section assume a bare fused silica capillary is used to perform the CE separations and that a DNA library is used.